https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26301 Wed 11 Apr 2018 14:11:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26480 Wed 11 Apr 2018 13:21:00 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30804 Wed 11 Apr 2018 12:10:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27739 Wed 11 Apr 2018 11:56:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30207 350 microRNAs (miRNAs), a developmentally important sRNA class, the majority (~60%) of which are also represented by the miRNA signature of spermatozoa. This includes >50 miRNAs that were found exclusively in epididymal sperm and epididymosomes, but not in the surrounding soma. We also documented substantial changes in the epididymosome miRNA cargo, including significant fold changes in almost half of the miRNAs along the length of the epididymis. Finally, we provide the first direct evidence for the transfer of several prominent miRNA species between mouse epididymosomes and spermatozoa to afford novel insight into a mechanism of intercellular communication by which the sRNA payload of sperm can be selectively modified during their post-testicular maturation.]]> Wed 11 Apr 2018 11:13:04 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38419 Wed 08 Sep 2021 16:10:39 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45607 Wed 02 Nov 2022 14:14:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35834 Kifc1 and Kifc5b, in aged oocytes; family members selectively targeted for expression regulation by endo-siRNAs of elevated abundance. The implications of reduced Kifc1 and Kifc5b expression were explored using complementary siRNA-mediated knockdown and pharmacological inhibition strategies, both of which led to increased rates of aneuploidy in otherwise healthy young oocytes. Collectively, our data raise the prospect that altered sRNA abundance, specifically endo-siRNA abundance, could influence the quality of the aged oocyte.]]> Tue 10 Dec 2019 16:34:23 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38608 Chlamydia is the most common sexually transmitted bacterial pathogen worldwide. Male and female infections occur at similar rates and both cause serious pathological sequelae. Despite this, the impact of chlamydial infection on male fertility has long been debated, and the effects of paternal chlamydial infection on offspring development are unknown. Using a male mouse chronic infection model, we show that chlamydial infection persists in the testes, adversely affecting the testicular environment. Infection increased leukocyte infiltration, disrupted the blood:testis barrier and reduced spermiogenic cell numbers and seminiferous tubule volume. Sperm from infected mice had decreased motility, increased abnormal morphology, decreased zona-binding capacity, and increased DNA damage. Serum anti-sperm antibodies were also increased. When both acutely and chronically infected male mice were bred with healthy female mice, 16.7% of pups displayed developmental abnormalities. Female offspring of chronically infected sires had smaller reproductive tracts than offspring of noninfected sires. The male pups of infected sires displayed delayed testicular development, with abnormalities in sperm vitality, motility, and sperm-oocyte binding evident at sexual maturity. These data suggest that chronic testicular Chlamydia infection can contribute to male infertility, which may have an intergenerational impact on sperm quality.]]> Thu 18 Nov 2021 11:59:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43344 Thu 15 Sep 2022 15:19:01 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32117 Thu 03 May 2018 12:04:54 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30490 Thu 03 Feb 2022 12:19:24 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35194 Thu 01 Aug 2019 17:06:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26158 Sat 24 Mar 2018 07:35:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22692 Kpna1, Kpna2, Kpna3, Kpna4, Kpna6, Kpna7, Kpnb1, Ipo5 and Xpo1), all were expressed in the embryonic ovary with up-regulation of protein levels concomitant with meiotic entry for KPNA2, accompanied by the redistribution of the cellular localisation of KPNA2 and XPO1. In contrast, postnatal folliculogenesis revealed significant up-regulation of Kpna1, Kpna2, Kpna4, Kpna6 and Ipo5 and down-regulation of Kpnb1, Kpna7 and Xpo1 at the primordial to primary follicle transition. KPNAs exhibited different localisation patterns in both oocytes and granulosa cells during folliculogenesis, with three KPNAs – KPNA1, KPNA2 and IPO5 – displaying marked enrichment in the nucleus by antral follicle stage. Remarkably, varied subcellular expression profiles were also identified in isolated pre-ovulatory oocytes with KPNAs KPNA2, KPNB1 and IPO5 detected in the cytoplasm and at the nuclear rim and XPO1 in cytoplasmic aggregates. Intriguingly, meiotic spindle staining was also observed for KPNB1 and XPO1 in meiosis II eggs, implying roles for KPNAs outside of nucleo-cytoplasmic transport. Thus, we propose that KPNAs, by targeting specific cargoes, are likely to be key regulators of oocyte development.]]> Sat 24 Mar 2018 07:11:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44774 Mon 24 Oct 2022 09:03:57 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54932 Fri 22 Mar 2024 14:34:29 AEDT ]]>